%0 Journal Article
%T Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells
%A Eun Jung Sohn
%A Hwan Tae Park
%A Kyoung-mi Bak
%A Yun-kyeong Nam
%J Archive of "Cancer Cell International".
%D 2018
%R 10.1186/s12935-018-0693-x
%X Cytotoxicity of curcumin in RT4 schwannoma cells. a Various concentrations of curcumin (0, 5, 10, 20, and 40 ¦̀M) were added to RT4 schwannoma cells and cell viability was assessed. b Various concentrations of Taxol (0, 5, 10, 20, and 40 ¦̀M) were added to RT4 cells and the MTT assay was performed. c Curcumin treatment (0, 10, or 20 ¦̀M) in RT4 schwannoma cells enhanced the expression of caspase-3, but attenuated proPARP, procaspase-3 and -9. Western blot analyses were performed with antibodies against PARP, caspase-3, caspase-9, and actin. Bar graphs represent the relative expression of proPARP, procaspase-9, or cleaved caspase-3 to ¦Â-actin determined using ImageJ software. d Curcumin treatment showed TUNEL-positive activity by microscopy. Fluorescent signals from fragmented DNA (green) and DAPI (blue) were visualized and photographed on a FLUOVIEW FV10i confocal microscope. Bar graph shows the quantitative analyses of apoptosis by the TUNEL assay. Data are presented as the mean£¿¡À£¿standard deviation (SD) of triplicate samples. ***p£¿<£¿0.001, **p£¿<£¿0.01, and *p£¿<£¿0.0
%K Curcumin
%K MicroRNA
%K Schwannoma
%K RT4
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278133/