%0 Journal Article
%T Mfn2 inhibits proliferation and cell-cycle in Hela cells via Ras-NF-¦ÊB signal pathway
%A Jian Yang
%A Jin She
%A Jun Hu
%A Jun Sun
%A Jun Yang
%A Kangquan Shou
%A Ping Yuan
%A Wenqi Gao
%A Xiaowen Liu
%A Yuanhong Zhou
%J Archive of "Cancer Cell International".
%D 2019
%R 10.1186/s12935-019-0916-9
%X The overexpression of Mfn2 and its effect on cell proliferation in Hela cells. a HeLa cells were incubated with Ad-Mfn2 (100 or 150pfu/cell) or Adv-control. The expression of Mfn2 mRNA in the Hela cells was detected by qRT-PCR. Data are represented as mean ¡À SD (*p£¿<£¿0.05, **p£¿<£¿0.01). b, c Cell lysate was extracted from HeLa cells treated with Adv-control, Adv-Mfn2 (100 or 150pfu/cell) for Mfn2 expression by western blot analysis (b) and quantitation (c). GAPDH was used as reference. d Adv-mfn2 were added to the medium of the cells, and incubated for 60 h. Cellular staining of nuclear DNA (DAPI) and localization of Mfn2 protein in HeLa cells. Magnification: 200¡Á. e, f HeLa cells were exposed to different concentrations of Adv-Mfn2 (0, 50, 100, 150 pfu/cell) for 48 h and 60 h, and then the cell viability was measured by CCK-8 assay. The Hela cells decreased after being treated with Adv-Mfn2 in a dose- and time-dependent manner. (mean ¡À SD from three independent experiments, **p£¿<£¿0.01). g After incubated with Adv-mfn2 (0, 100 and 150 pfu/cell), the expression of PCNA protein in HeLa cells was detected by western blot. ¦Â-actin was used as referenc
%K Mfn2
%K Ras
%K Cervical carcinoma
%K Cell proliferation
%K Cell-cycle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664827/