%0 Journal Article
%T Clinical implications of serum Nİ\glycan profiling as a diagnostic and prognostic biomarker in germİ\cell tumors
%A Chikara Ohyama
%A Hayato Yamamoto
%A Ippei Takahashi
%A Koji Mitsuzuka
%A Norihiko Tsuchiya
%A Sadafumi Kawamura
%A Shigeyuki Nakaji
%A Shingo Hatakeyama
%A Shinichi Yamashita
%A Shintaro Narita
%A Takuma Narita
%A Takuya Koie
%A Tatsuo Tochigi
%A Tohru Yoneyama
%A Tomonori Habuchi
%A Toshihiko Sakurai
%A Yoichi Arai
%A Yuki Tobisawa
%J Archive of "Cancer Medicine".
%D 2017
%R 10.1002/cam4.1035
%X Serum biomarker monitoring is essential for management of germİ\cell tumors (GCT). However, not all GCT are positive for conventional tumor markers. We examined whether serum Nİ\glycanİ\based biomarkers can be applied for detection and prognosis in patients with GCT. We performed a comprehensive Nİ\glycan structural analysis of sera from 54 untreated GCT patients and 103 ageİ\adjusted healthy volunteers using glycoblotting methods and mass spectrometry. Candidate Nİ\glycans were selected from those with the highest association; cutoff concentration values were established, and an Nİ\glycan score was created based on the number of positive Nİ\glycans present. The validity of this score for diagnosis and prognosis was analyzed using a receiver operating characteristic (ROC) curve. We identified five candidate Nİ\glycans significantly associated with GCT patients. The accuracy of the Nİ\glycan score for GCT was significant with an areaİ\underİ\theİ\curve (AUC) value of 0.87. Diagnostically, the Nİ\glycan score detected 10 of 12 (83%) patients with negative conventional tumor markers. Prognostically, the Nİ\glycan score comprised four candidate Nİ\glycans. The predictive value of the prognostic Nİ\glycan score was significant, with an AUC value of 0.89. A high value prognostic Nİ\glycan score was significantly associated with poor prognosis. Finally, to identify a potential carrier protein, immunoglobulin (Ig) fractions of sera were subjected to Nİ\glycan analysis and compared to whole sera. Candidate Nİ\glycans in Igİ\fractions were significantly decreased; therefore, the carrier protein for candidate Nİ\glycans is likely not an immunoglobulin. In summary, our newly developed Nİ\glycan score seems to be a practical diagnostic and prognostic method for GCT
%K Biomarker
%K germİ\cell tumors
%K glycoblotting
%K mass spectrometry
%K serum Nİ\glycan
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387168/