%0 Journal Article
%T Downregulation of miRNAİ\141 in breast cancer cells is associated with cell migration and invasion: involvement of ANP32E targeting
%A Guolian Pang
%A Jiankun Zhang
%A Kuichun Zhu
%A Liangying Liao
%A Lu Han
%A Ping Li
%A Tao Xu
%A Wan Luo
%A Xianyong Luo
%A Xiaobing Xie
%A Xin Zhou
%J Archive of "Cancer Medicine".
%D 2017
%R 10.1002/cam4.1024
%X MicroRNAs (miRNAs) regulate many cellular activities, including cancer development, progression, and metastasis. Some miRNAs are involved in breast cancer (BC) migration and invasion, thus affect patientsĦŻ prognosis. Microarray analysis was performed to compare miRNA expression in BC tissues, and results confirmed by qPCR. BC cell migration and invasion were studied in vitro with MDAİ\MBİ\231 cells using microplate transwell assays. miRNA targeting was investigated using luciferase assays, qPCR, and Western blot analysis in cells with overexpression of miRNA mimics. Knockdown of miRNA targets was performed using target siRNA lentiviral infection. Results show that microRNAİ\141 (miRİ\141) was downregulated in breast cancer tumor tissues compared with matched surrounding tissues. Downregulation of miRİ\141 expression correlated with tumor stage, lymph node involvement, and expressions of PCNA, Ki67, and HER2. Overexpression of miRİ\141 inhibited BC cell proliferation, migration, and invasion in vitro. ANP32E gene was selected as one putative target for further studies based on results from in silico analysis. Results from a dualİ\luciferase reporter system suggested ANP32E as a direct target of miRİ\141. Overexpression of miRİ\141 downregulated ANP32E expression at both mRNA and protein levels in BC cells. Knockdown of ANP32E inhibited BC cell proliferation, migration, and invasion in vitro, mimicking the effect of the overexpression of miRİ\141. Our study revealed important roles miRİ\141 plays in BC growth and metastasis. Moreover, for the first time, we identified ANP32E as one of the miRİ\141 targets, and demonstrated its involvement in the regulation of cell proliferation, migration, and invasion
%K ANP32E
%K breast cancer
%K invasion
%K migration
%K miRİ\141
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345683/