%0 Journal Article
%T Effects of SAHA and EGCG on Growth Potentiation of Triple-Negative Breast Cancer Cells
%A Harrison R. Jordan
%A Kayla A. Lewis
%A Trygve O. Tollefsbol
%J Archive of "Cancers".
%D 2019
%R 10.3390/cancers11010023
%X Triple-negative breast cancer comprises approximately 15¨C20% of all breast cancers diagnosed and is nearly twice as common in black women than white women in the United States. We evaluated the effects of two epigenetic-modifying compounds on markers of growth potential in several triple-negative breast cancer cell lines. Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor currently used in the treatment of cutaneous T cell lymphoma, was administered to triple-negative breast cancer cells alone or in combination with epigallocatechin-3-gallate (EGCG), a DNA methyltransferase (DNMT) inhibitor isolated from green tea. The compounds affected the expression of oncogenic miR-221/222 and tumor suppressors, p27 and PTEN, in addition to estrogen receptor alpha (ER¦Á). E-cadherin expression was increased while N-cadherin was decreased, indicating a more epithelial phenotype. In addition, the activity of DNMTs was diminished with the treatments, and there was a significant enrichment of AcH3 within the promoter of p27 and PTEN, suggesting a role of epigenetic mechanisms for the aforementioned changes. These results translated to reduced migration of the triple-negative breast cancer cells with the treatments. Together, these findings support the role of SAHA and EGCG in limiting growth and proliferation of breast cancer cells
%K Mesenchymal-to-epithelial transition
%K breast cancer
%K DNMT inhibitors
%K HDAC inhibitors
%K phytochemicals
%K microRNA
%K cancer epigenetics
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356328/