%0 Journal Article
%T T cell receptor β‐chain repertoire analysis of tumor‐infiltrating lymphocytes in pancreatic cancer
%A Bin Dong
%A Can Cui
%A Chaoting Zhang
%A Chunyi Hao
%A Jianhui Wu
%A Min Zhao
%A Qin Tan
%A Xiaoya Guan
%A Xiuyun Tian
%A Zheming Lu
%J Archive of "Cancer Science".
%D 2019
%R 10.1111/cas.13877
%X Pancreatic cancer is lethal due to lack of perceptible symptoms and effective treatment methods. Immunotherapy may provide promising therapeutic choices for malignant tumors like pancreatic cancer. Tumor‐infiltrating lymphocytes (TIL) in tumor mesenchyme could recognize peptide antigens presented on the surface of tumor cells. The present study aimed to test the relationship between the T cell receptor (TCR) β repertoire of the tumor and peripheral blood, and also to investigate the intra‐tumor spatial heterogeneity of the TCR β repertoire in pancreatic cancer. To the best of our knowledge, this is the first study to evaluate the clonal composition of TCR β repertoire in TIL across the spatial extent of pancreatic cancer. In this study, we studied 5 patients who were diagnosed with primary pancreatic cancer. Ultra‐deep sequencing was used to assess the rearrangement of the TCR β‐chain (TCR β) gene. HE staining and immunohistochemistry of CD3, CD4, CD8 and HLA class I were used to show histopathology and immune conditions macroscopically. TIL repertoire showed that different regions of the same tumor showed a greater number of repertoire overlaps between each other than between peripheral blood, which suggested that T cell clones in pancreatic cancer might be quite different from those in peripheral blood. In contrast, intra‐tumoral TCR β repertoires were spatially homogeneous between different regions of a single tumor tissue. Based on these results, we speculated that the cellular adaptive immune response in pancreatic cancer was spatially homogeneous; this may pave the way for immunotherapy for the treatment of pancreatic cancer patients
%K immunohistochemistry
%K intra‐tumor spatial heterogeneity
%K pancreatic cancer
%K T cell receptor
%K ultra‐deep sequencing
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317932/