%0 Journal Article
%T Development of a T©\cell receptor multimer with high avidity for detecting a naturally presented tumor©\associated antigen on osteosarcoma cells
%A Hidekazu Kameshima
%A Kazue Watanabe
%A Munehide Nakatsugawa
%A Noriyuki Sato
%A Shingo Toji
%A Shogo Saitoh
%A Takayuki Kanaseki
%A Takeshi Terui
%A Terufumi Kubo
%A Tomohide Tsukahara
%A Toshihiko Torigoe
%A Yoshihiko Hirohashi
%J Archive of "Cancer Science".
%D 2019
%R 10.1111/cas.13854
%X For efficacy of peptide vaccination immunotherapy for patients with cancer, endogenous expression of the target peptide/human leukocyte antigen (HLA) on cancer cells is required. However, it is difficult to evaluate the expression status of a peptide/HLA complex because of the lack of a soluble T©\cell receptor (TCR) that reacts with tumor©\associated antigen (TAA) with high avidity. In the present study, we developed two soluble TCR©\multimers that were each directed to TAA, survivin©\2B (SVN©\2B) and PBF in the context of HLA©\A24 (SVN©\2B TCR©\multimer and PBF TCR©\multimer, respectively), from CTL clones that were established from peptide©\vaccinated patients. Both TCR multimers could recognize cognate peptide©\pulsed antigen©\presenting cells, C1R©\A24 cells, in a CD8©\independent method. Moreover, the PBF TCR©\multimer successfully recognized a PBF peptide naturally presented on HLA©\A24+ PBF + osteosarcoma cells. Taken together, the results indicated that a TCR©\multimer might be useful for detection of a TAA©\derived peptide presented by HLA in patients receiving immunotherapy
%K HLA©\A24
%K multimer
%K PBF
%K survivin
%K T©\cell receptor
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317924/