%0 Journal Article
%T Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways
%A Hina Mir
%A Ian T. Saunders
%A Neeraj Kapur
%A Shailesh Singh
%J Archive of "Cancer Cell International".
%D 2019
%R 10.1186/s12935-019-0820-3
%X Emodin reduces the cell viability and induces apoptosis in human colon cancer cells. Colon epithelial cells (CCD 841 CoN) and colon cancer (CoCa) cells (DLD-1 and COLO 201) were treated with increasing concentrations of Emodin (0¨C80 ¦ÌM) for 24 h (white bar) 48 h (grey bar) and 72 h (black bar). MTT assay was used to measure cell viability and EC50 was determined via a linear regression (a). DLD-1 and COLO 201 cells were treated with EC50 of Emodin 18 ¦ÌM and 15 ¦ÌM, respectively and apoptosis was assessed by staining with FITC-conjugated Annexin V and 7-AAD (b). The percentage of necrotic, late and early apoptotic and non-apoptotic cells are shown in Q1, Q2, Q3 and Q4. c Bar diagrams show percentage changes in necrotic, late and early apoptotic, and non-apoptotic cells as mean£¿¡À£¿SEM from three independent experiments. Asterisks (*) p£¿<£¿0.05, (**) p£¿<£¿0.01, (***) p£¿<£¿0.001, show significant change compared with the contro
%K Colon cancer
%K Emodin
%K Anthraquinone
%K Apoptosis
%K Mitochondria
%K Bcl-2
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466701/