%0 Journal Article %T Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues %A Akira Hara %A Ayumi Niwa %A Hidenori Ito %A Hidenori Tabata %A Hitomi Aoki %A Ikuko Iwamoto %A Koh-ichi Nagata %A Kyoko Ibaraki %A Makoto Mizuno %J Archive of "Acta Histochemica et Cytochemica". %D 2018 %R 10.1267/ahc.18009 %X ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a useful tool for biochemical and cell biological analyses of ARHGEF9 %K ARHGEF9 %K guanine nucleotide exchange factor (GEF) %K antibody %K cerebral cortex %K neuron %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066644/