%0 Journal Article
%T Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
%A Joachim J.C. Potgieter
%A Marleen Kock
%A Piet Becker
%A Tshiphiri Senamela
%J Archive of "African Journal of Laboratory Medicine".
%D 2018
%R 10.4102/ajlm.v7i1.658
%X The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806058/