%0 Journal Article
%T Calmodulin Polymerase Chain Reaction¨CRestriction Fragment Length Polymorphism for Leishmania Identification and Typing
%A Aracelis Miranda
%A Azael Salda£¿a
%A Franklyn Samudio
%A Jose E. Calzada
%A Kadir Gonz¨¢lez
%J Archive of "The American Journal of Tropical Medicine and Hygiene".
%D 2016
%R 10.4269/ajtmh.15-0709
%X A precise identification of Leishmania species involved in human infections has epidemiological and clinical importance. Herein, we describe a preliminary validation of a restriction fragment length polymorphism assay, based on the calmodulin intergenic spacer region, as a tool for detecting and typing Leishmania species. After calmodulin amplification, the enzyme HaeIII yielded a clear distinction between reference strains of Leishmania mexicana, Leishmania amazonensis, Leishmania infantum, Leishmania lainsoni, and the rest of the Viannia reference species analyzed. The closely related Viannia species: Leishmania braziliensis, Leishmania panamensis, and Leishmania guyanensis, are separated in a subsequent digestion step with different restriction enzymes. We have developed a more accessible molecular protocol for Leishmania identification/typing based on the exploitation of part of the calmodulin gene. This methodology has the potential to become an additional tool for Leishmania species characterization and taxonomy
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973186/