%0 Journal Article
%T Immunohistochemical Study of the Laminin ¦Á5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands
%A Alimuddin Tofrizal
%A Depicha Jindatip
%A Fujianti Casmad
%A Ken Fujiwara
%A Khongorzul Batchuluun
%A Morio Azuma
%A Motoshi Kikuchi
%A Nobuhiko Ohno
%A Rahimi Syaidah
%A Rita Maliza
%A Takehiro Tsukada
%A Takeshi Inagaki
%J Archive of "Acta Histochemica et Cytochemica".
%D 2018
%R 10.1267/ahc.18014
%X Laminin, a major basement membrane protein, comprises three subunit chains: ¦Á, ¦Â, and ¦Ã chains. Among these chains, only the laminin ¦Á chain is capable of signaling via laminin receptors. Although laminin isoforms containing the ¦Á5 chain were reported to be the first laminin produced during rat anterior pituitary gland development, the functions of these isoforms are unknown. We used immunohistochemical techniques to localize the laminin ¦Á5 chain and its specific receptor, basal cell adhesion molecule (BCAM), in fetal and adult pituitary gland. Laminin ¦Á5 chain immunoreactivity was observed in the basement membrane of the primordial adenohypophysis at embryonic days 12.5 to 19.5. Double immunostaining showed that BCAM was present and co-localized with the laminin ¦Á5 chain in the tissue. Quantitative analysis showed that the laminin ¦Á5 chain and BCAM were expressed in the anterior pituitary gland during postnatal development and in adulthood (postnatal day 60). In the adult gland, co-localization of the laminin ¦Á5 chain and BCAM was observed, and BCAM was detected in both the folliculo-stellate cells and endothelial cells. These results suggest that laminin ¦Á5 chain signaling via BCAM occurs in both the fetal adenohypophysis and adult anterior pituitary gland
%K laminin
%K basal cell adhesion molecule
%K laminin receptor
%K pituitary development
%K immunohistochemistry
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261841/