%0 Journal Article %T Genome Editing for Cancer Therapy: Delivery of Cas9 Protein/sgRNA Plasmid via a Gold Nanocluster/Lipid Core¨CShell Nanocarrier %A Lingmin Zhang %A Nuoxin Wang %A Peng Wang %A Rongbing Tang %A Wenfu Zheng %A Yangzhouyun Xie %J Archive of "Advanced Science". %D 2017 %R 10.1002/advs.201700175 %X The type II bacterial clustered, regularly interspaced, short palindromic repeats (CRISPR)©\Cas9 (CRISPR©\associated protein) system (CRISPR©\Cas9) is a powerful toolbox for gene©\editing, however, the nonviral delivery of CRISPR©\Cas9 to cells or tissues remains a key challenge. This paper reports a strategy to deliver Cas9 protein and single guide RNA (sgRNA) plasmid by a nanocarrier with a core of gold nanoclusters (GNs) and a shell of lipids. By modifying the GNs with HIV©\1©\transactivator of transcription peptide, the cargo (Cas9/sgRNA) can be delivered into cell nuclei. This strategy is utilized to treat melanoma by designing sgRNA targeting Polo©\like kinase©\1 (Plk1) of the tumor. The nanoparticle (polyethylene glycol©\lipid/GNs/Cas9 protein/sgPlk1 plasmid, LGCP) leads to >70% down©\regulation of Plk1 protein expression of A375 cells in vitro. Moreover, the LGCP suppresses melanoma progress by 75% on mice. Thus, this strategy can deliver protein©\nucleic acid hybrid agents for gene therapy %K cancer therapy %K CRISPR/Cas9 %K genome editing %K gold nanoclusters %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700650/