%0 Journal Article
%T Oneİ\Step Generation of a Drugİ\Releasing Hydrogel Microarrayİ\Onİ\Aİ\Chip for Largeİ\Scale Sequential Drug Combination Screening
%A Amos Chungwon Lee
%A Daewon Lee
%A Dong Yoon Oh
%A Hyung Jong Bae
%A Jiyun Kim
%A Seo Woo Song
%A Su Deok Kim
%A Sumin Lee
%A Yongju Lee
%A Yunjin Jeong
%J Archive of "Advanced Science".
%D 2019
%R 10.1002/advs.201801380
%X Largeİ\scale screening of sequential drug combinations, wherein the dynamic rewiring of intracellular pathways leads to promising therapeutic effects and improvements in quality of life, is essential for personalized medicine to ensure realistic cost and time requirements and less sample consumption. However, the largeİ\scale screening requires expensive and complicated liquid handling systems for automation and therefore lowers the accessibility to clinicians or biologists, limiting the full potential of sequential drug combinations in clinical applications and academic investigations. Here, a miniaturized platform for highİ\throughput combinatorial drug screening that is Ħ°pipettingİ\freeĦħ and scalable for the screening of sequential drug combinations is presented. The platform uses parallel and bottomİ\up formation of a heterogeneous drugİ\releasing hydrogel microarray by selfİ\assembly of drugİ\laden hydrogel microparticles. This approach eliminates the need for liquid handling systems and timeİ\consuming operation in highİ\throughput largeİ\scale screening. In addition, the serial replacement of the drugİ\releasing microarrayİ\onİ\aİ\chip facilitates different drug exchange in each and every microwell in a simple and highly parallel manner, supporting scalable implementation of multistep combinatorial screening. The proposed strategy can be applied to various forms of combinatorial drug screening with limited amounts of samples and resources, which will broaden the use of the largeİ\scale screening for precision medicine
%K drugİ\laden hydrogel
%K encoded microparticle
%K highİ\throughput screening
%K selfİ\assembly
%K sequential combination
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364496/