%0 Journal Article
%T MiR-654-5p attenuates breast cancer progression by targeting EPSTI1
%A Cheng-Wu Zhang
%A Jin-Feng Wang
%A Sheng-Chu Zhang
%A Xiao-Yun Xu
%A Yu-Yan Tan
%J Archive of "American Journal of Cancer Research".
%D 2016
%X MicroRNAs (miRNAs) dysregulation is a common event in a variety of human diseases including breast cancer. However, clinical relevance and biological role of miR-654-5p in the progression of breast cancer remain greatly elusive. Herein, the expression levels of miR-654-5p were aberrantly downregulated in human breast cancer specimens and four breast cancer cell lines. Low expression of miR-654-5p was strongly associated with advanced TNM stage and lymph node metastasis as well as a poor survival. Functional analysis showed that miR-654-5p overexpression inhibited cell growth and invasion, and induced cell apoptosis in two aggressive breast cancer cells. Further studies demonstrated that Epithelial stromal interaction 1 (EPSTI1) was a direct target gene of miR-654-5p and showed an inverse correlation with miR-654-5p expression. Forced expression of EPSTI1 could abrogate the inhibitory effect of miR-654-5p on the growth and invasion of breast cancer cells as well as apoptosis-induced ability. In conclusion, the present study highlights that miR-654-5p acts as a tumor suppressor in breast cancer through directly targeting EPSTI1, and their functional regulation may open a novel avenue with regard to the therapeutic target for breast cancer
%K miRNA
%K breast cancer
%K miR-654-5p
%K EPSTI1
%K survival
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859678/