%0 Journal Article
%T Development of a SYBR Green I real-time PCR for the detection of the orf virus
%A Caixia Bai
%A Dongdong Yin
%A Guijun Wang
%A Kankan Yang
%A Kezong Qi
%A Yong Wang
%A Yongdong Li
%J Archive of "AMB Express".
%D 2017
%R 10.1186/s13568-016-0322-9
%X Orf is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). ORFV occasionally causes cutaneous lesions in humans in contact with infected animals. In the present study, a real-time PCR method was established for detection of ORFV using the fluorescent chimeric dye SYBR Green I. Specific primers were designed to target a highly conserved region of the ORFV B2L gene. This method was able to detect a minimum of 20 copies of ORFV genomic DNA. The results showed no cross-reactions with other common DNA viruses. The time required for the test was approximately 1.5 h. Clinical test samples showed that this method was faster and had a higher sensitivity than traditional PCR. In conclusion, this novel, real-time PCR-based assay provides a rapid, sensitive, and specific method for ORFV detection. This test provides improved technical support for studies regarding the clinical diagnosis and epidemiology of ORFV
%K Detection
%K Orf virus
%K Real-time PCR
%K SYBR Green I
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218949/