%0 Journal Article
%T Intestinal uptake of barley protein-based nanoparticles for ¦Â-carotene delivery
%A Guangyu Liu
%A Ying Zhou
%J Archive of "Acta Pharmaceutica Sinica. B".
%D 2019
%R 10.1016/j.apsb.2018.10.002
%X Our previous study introduced a barley protein microparticle for encapsulation of hydrophobic drug/nutraceutical, which could release nanoparticles upon gastric digestion and deliver encapsulated compound to a simulated intestinal environment intact. This work focused on evaluating the potential of liberated nanoparticles to improve the absorption of encapsulated compounds (e.g., ¦Â-carotene) using in vitro Caco-2 cell and ex vivo small intestine models. Nanoparticles obtained from gastric digestion of barley protein microparticles had a spherical shape and an average size of 351£¿nm. Nanoparticles showed low cytotoxicity in Caco-2 cells and their cellular uptake was dependent on time, concentration and temperature. In a Caco-2 cell monolayer model, significantly greater uptake and transport of ¦Â-carotene were observed when it was delivered by nanoparticles (15%), compared to free ¦Â-carotene suspension (2.6%). In an ex vivo rat jejunum model, nanoparticles showed the capacity to retain in small intestinal tissue. Approximately 2.24 and 6.04£¿¦Ìg nanoparticle were able to permeate through each cm2 intestinal tissue and translocate to the serosal side after 60 and 90£¿min, respectively. Results from this study demonstrated the absorption improving effect of the barley protein nanoparticles and suggested their potential as vehicles for hydrophobic compounds
%K Barley protein
%K Nanoparticles
%K Hydrophobic
%K Caco-2 cell
%K Intestinal transport
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362262/