%0 Journal Article
%T Bioinspired Preservation of Natural Killer Cells for Cancer Immunotherapy
%A Alessandro Tocchio
%A Claudia Zylberberg
%A David L. Kaplan
%A Lotfi Abou©\Elkacem
%A Rami El Assal
%A Sandro Matosevic
%A Shannon Pasley
%J Archive of "Advanced Science".
%D 2019
%R 10.1002/advs.201802045
%X The ability to cryopreserve natural killer (NK) cells has a significant potential in modern cancer immunotherapy. Current cryopreservation protocols cause deterioration in NK cell viability and functionality. This work reports the preservation of human cytokine©\activated NK cell viability and function following cryopreservation using a cocktail of biocompatible bioinspired cryoprotectants (i.e., dextran and carboxylated ¦Å©\poly©\L©\lysine). Results demonstrate that the recovered NK cells after cryopreservation and rewarming maintain their viability immediately after thawing at a comparable level to control (dimethyl sulfoxide©\based cryopreservation). Although, their viability drops in the first day in culture compared to controls, the cells grow back to a comparable level to controls after 1 week in culture. In addition, the anti©\tumor functional activity of recovered NK cells demonstrates higher cytotoxic potency against leukemia cells compared to control. This approach presents a new direction for NK cell preservation, focusing on function and potentially enabling storage and distribution for cancer immunotherapy
%K bioinspired materials
%K biopreservation
%K cryoprotectants
%K immunotherapy
%K natural killer cells
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425501/