%0 Journal Article
%T Histopathological evaluation of mesenchymal stem cells in the healing of anastomosed carotid arteries
%A Abdullah Hilmi Marangoz
%A Adnan Altun
%A Cengiz £¿okluk
%A Enis Kuruo£¿lu
%A Ercan Yarar
%A Ey¨¹p Gen£¿
%A Keramettin Ayd£¿n
%A Levent Y£¿ld£¿z
%A Sezgin Gunes
%J Archive of "American Journal of Stem Cells".
%D 2019
%X The objective of this study was to evaluate the influence of mesenchymal stem cells on the healing of experimental carotid artery anastomoses histopathologically. Twenty-four female Sprague-Dawley rats were used in this study. After random separation of the subjects into two groups, in both groups carotid arteries were transected and anastomosed in end-to-end fashion. Anastomoses were locally treated with 1 ml 0.09% NaCl, and 1 ml mesenchymal stem cell suspension (1¡Á106 cells) in control and trial groups, respectively. Anastomoses were wrapped with an 8 mm sheet of surgicel and soaked with BioGlue in order to sequestrate the stem cells. After patencies were confirmed via Doppler USG, surgical site was closed with 2/0 silk sutures. Histopathological evaluation was carried out after 4 weeks. In respect to endothelial continuity, vessel patency (along with presence or absence of restenosis), integrities of internal and external elastic laminae, muscularis and adventitia; no statistically significant differences were present between the trial and control groups. In Trial and Control Groups, luminal thrombus was present in 8 (66.6%) and 3 (25%) of the 12 subjects, respectively. The difference was statistically significant (P < 0.05). Recanalization was present in 6 subjects in trial group; 1 subjects in Control Group, respectively. Our results suggest that local administration of mesenchyme stem cell does not have a positive influence on success of an anastomosis
%K End to end carotid arteries anastomosis
%K local application of mesenchyme stem cells
%K histopathological evaluation
%K thrombosis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526360/