%0 Journal Article
%T Tuberculosis: Bacteriological And Epidemiological Aspects in The Central Region of Tunisia SciDoc Publishers | Open Access | Science Journals | Media Partners
%A Hechmi Kenani
%A Moncef Ben Said
%A Asma Ferjani
%A Hasna Tarmiz
%A Imen Ben Kahla
%A JalelBoukadida
%A Manel Marzouk
%A Nadia Gharbi
%A Walid Ben Salma
%J Microbiology & Advanced Immunology (IJMAI)
%D 2018
%R http://dx.doi.org/10.19070/2329-9967-130003
%X It is a retrospective study conducted in the coastal region of east-central Tunisia, about 500 000 people with a socio-economic development among the highest in Tunisia and an annual income per capita of 4200 dollars. The national incidence of TB in Tunisia was 20.6 (in 2009) [1]. The study included all TB cases diagnosed between January 1st, 2006 and December 31, 2009 on the basis of biological and / or clinical or histo-pathological criteria. Patients files collected were: sex, age, origin, marital status, socioeconomic status, clinical characteristics (including past history of TB, diabetes mellitus, malignancies, HIV...), treatment history (use of immunosuppressive drugs, steroids), past history of treatment for TB, smoking habits, history of known contact with a case of TB, stay in closed community (prison), date of hospital admission, hospital department, symptoms brought patient to consult (fever, weight loss, sweats, cough ..), chest radiographs investigations and the delay between the onset of symptoms and TB diagnosis (date of commencing treatment). The bacteriological study included: the number of samples examined per patient and kind of each one (sputum, bronchial fluid, gastric aspirate, lymph node, pleural effusion...), date of sampling, result of Ziehl. Neelsen staining (ZNS) (noted depending on the richness of acid fast bacilli (AFB) notified by number of crosses), result of cultures and positivity delay, and susceptibility to the first line agents (rifampicin, isoniazid (INH), streptomycin, ethambutol, and / or pyrazinamide). The pulmonary samples were inoculated on L£¿wenstein Jensen (LJ) culture medium. The extra-pulmonary samples were inoculated on solid (LJ and Coletsos) and liquid (BacT/Alert MB; BioM¨¦rieux, France) culture media. Culture media were incubated at 37 ¡ã C in an inclined position up to 3 months for solid media and 40 days for liquid media. Cultures on solid medium were examined once a week, those on liquid medium considered to the alarm signal. Bacteria grown on culture media were identified by conventional biochemical techniques [2] and confirmed by the GenoType molecular kits (Hain Lifescience GmbH, Nehren, Germany). Susceptibility to the first line drugs was testing by the technique of proportions (Biorad). Patients with extended disease to organs or tissues outside the thorax, including those patients who had pulmonary involvement, were considered as extra-pulmonary TB (EPTB)
%K n/a
%U https://scidoc.org/IJMAI-2329-9967-01-202.php