%0 Journal Article
%T Expression of Tropomyosin 2 Gene Isoforms in Human Cardiac Tissue
%A Bernard J Poiesz
%A Dipak K Dube
%A Joseph Lachant
%A Lynn Abbott
%A Patricia Benz
%A Santhi Yalamanchili
%A Syamalima Dube
%J Cardiology and Research (IJCRR)
%D 2018
%R http://dx.doi.org/10.19070/2470-4563-140003
%X Previous studies have shown that although the transcript levels of TPM1¦Á and TPM1¦Ê are expressed in human hearts in comparable levels, the level of TPM1¦Á protein is ~90%. The proteins of TPM1¦Ê and TPM2¦Á are about 5% of the total sarcomeric TM. The TPM2 gene is known to generate three alternatively spliced isoforms, which are designated as TPM2¦Á TPM2¦Â TPM2¦Ã. The expression level of TPM2¦Â and TPM2¦Ã in human hearts is unknown. Using a series of primers pairs and probes for RNA PCR, we found that both TPM2¦Á and ¦Â but not ¦Ã were expressed in fetal and adult heart tissue, with about the same amounts of each isoform in fetal hearts and more ¦Â than ¦Á in adult hearts. Four new isoforms of TPM2 RNA were identified (TPM2¦Ä-¦Ç). Most of these were present in very small amounts in both the fetal and adult hearts with the exception of TPM2¦Î, which was present at about 40% of the level of TPM2¦Á in adult heart tissue. Western blot analyses using a series of anti-tropomyosin antibodies indicate that TPM2 protein is present in both fetal and adult hearts at about the same levels as TPM1¦Ê and much less than TPM1¦Á. We are unsure about the expression of TPM2¦Ä, TPM2¦Æ, and TPM2¦Ç proteins in fetal and adult human hearts. The exact function of these new TPM2 isoforms in heart and their role(s) in cardiac disease remain to be elucidated
%K n/a
%U https://scidoc.org/IJCRR-2470-4563-01-301.php