%0 Journal Article
%T PKM¦Æ, But Not PKC¦Ë, Is Rapidly Synthesized and Degraded at the Neuronal Synapse
%A John T. Ngo
%A Larry A. Gross
%A Margaret T. Butko
%A Mark H. Ellisman
%A Mason R. Mackey
%A Roger Y. Tsien
%A Sakina F. Palida
%J The Journal of Neurosience
%D 2015
%R 10.1523/JNEUROSCI.0004-15.2015
%X Synthesizing, localizing, and stabilizing new protein copies at synapses are crucial factors in maintaining the synaptic changes required for storing long-term memories. PKM¦Æ recently emerged as a molecule putatively responsible for maintaining encoded memories over time because its presence correlates with late LTP and because its inhibition disrupts LTP in vitro and long-term memory storage in vivo . Here we investigated PKM¦Æ stability in rat neurons to better understand its role during information encoding and storage. We used TimeSTAMP reporters to track the synthesis and degradation of PKM¦Æ as well as a related atypical PKC, PKC¦Ë. These reporters revealed that both PKM¦Æ and PKC¦Ë were upregulated after chemical LTP induction; however, these new PKM¦Æ copies exhibited more rapid turnover than basally produced PKM¦Æ, particularly in dendritic spines. In contrast to PKM¦Æ, new PKC¦Ë copies exhibited elevated stability. Stable information storage over long periods of time is more challenging the shorter the metabolic lifetime of the candidate molecules
%U http://www.jneurosci.org/content/35/20/7736