%0 Journal Article
%T Cross-Recognition of a Myelin Peptide by CD8+ T Cells in the CNS Is Not Sufficient to Promote Neuronal Damage
%A Alexander U. Brandt
%A Eva Reuter
%A Frauke Zipp
%A H¨¦l¨¨ne Salmon
%A J¨¦r£¿me Birkenstock
%A Magdalena Paterka
%A Nadia Grohmann
%A Ren¨¦ Gollan
%A Sebastian Richers
%A Tanja Kuhlmann
%A Tina Leuenberger
%A Volker Siffrin
%J The Journal of Neurosience
%D 2015
%R 10.1523/JNEUROSCI.3380-14.2015
%X Multiple sclerosis (MS) is an inflammatory disease of the CNS thought to be driven by CNS-specific T lymphocytes. Although CD8+ T cells are frequently found in multiple sclerosis lesions, their distinct role remains controversial because direct signs of cytotoxicity have not been confirmed in vivo . In the present work, we determined that murine ovalbumin-transgenic (OT-1) CD8+ T cells recognize the myelin peptide myelin oligodendrocyte glycoprotein 40¨C54 (MOG40¨C54) both in vitro and in vivo . The aim of this study was to investigate whether such cross-recognizing CD8+ T cells are capable of inducing CNS damage in vivo . Using intravital two-photon microscopy in the mouse model of multiple sclerosis, we detected antigen recognition motility of the OT-1 CD8+ T cells within the CNS leading to a selective enrichment in inflammatory lesions. However, this cross-reactivity of OT-1 CD8+ T cells with MOG peptide in the CNS did not result in clinically or subclinically significant damage, which is different from myelin-specific CD4+ Th17-mediated autoimmune pathology. Therefore, intravital imaging demonstrates that local myelin recognition by autoreactive CD8+ T cells in inflammatory CNS lesions alone is not sufficient to induce disability or increase axonal injury
%U http://www.jneurosci.org/content/35/12/4837