%0 Journal Article
%T Production of Ligninolytic Enzymes by White-Rot Fungus Datronia sp. KAPI0039 and Their Application for Reactive Dye Removal
%A Pilanee Vaithanomsat
%A Waraporn Apiwatanapiwat
%A Oncheera Petchoy
%A Jirawate Chedchant
%J International Journal of Chemical Engineering
%D 2010
%I Hindawi Publishing Corporation
%R 10.1155/2010/162504
%X This study focused on decolorization of 2 reactive dyes; Reactive Blue 19 (RBBR) and Reactive Black 5 (RB5), by selected white-rot fungus Datronia sp. KAPI0039. The effects of reactive dye concentration, fungal inoculum size as well as pH were studied. Samples were periodically collected for the measurement of color unit, Laccase (Lac), Manganese Peroxidase (MnP), and Lignin Peroxidase (LiP) activity. Eighty-six percent of 1,000£¿ RBBR decolorization was achieved by 2% (w/v) Datronia sp. KAPI0039 at pH 5. The highest Lac activity (759.81£¿ ) was detected in the optimal condition. For RB5, Datronia sp. KAPI0039 efficiently performed (88.01% decolorization) at 2% (w/v) fungal inoculum size for the reduction of 600£¿ RB5 under pH 5. The highest Lac activity (178.57£¿ ) was detected, whereas the activity of MnP and LiP was absent during this hour. The result, therefore, indicated that Datronia sp. KAPI0039 was obviously able to breakdown both reactive dyes, and Lac was considered as a major lignin-degradation enzyme in this reaction. 1. Introduction Large amount of chemical dyes, approximately 10,000 different dyes and pigments per year, are used for various industrial applications such as textile and printing industries. It is estimated that about 10% are lost in industrial effluents [1]. As a result, a significant proportion of these dyes are released to the environment in wastewater. Moreover, these dyes are designed to be resistant to light, water, and oxidizing agents and therefore difficult to naturally degrade once released into aquatic systems [2]. Thus, this can cause the obstruction of sunlight pass through the water resource by synthetic dyes, then leading to the decrease in oxygen dissolved in water, the photosynthesis of water plants, and the biodegradation of organic matters. At present, the biotechnological approaches were proven to be potentially effective in treatment of this pollution source in an eco-efficient manner [2]. The possibility to use ligninolytic fungi for the removal of synthetic dyes is one approach that attracts considerable attention. This is due to their production of ligninolytic enzymes¡ªmost frequently laccase and manganese peroxidase¡ªthat enable these microorganisms to oxidize a broad range of substrates including synthetic dyes [3]. Many reports so far have demonstrated that white-rot fungi, such as Phanerochaete chrysosporium, Trametes versicolor, Pleurotus ostreatus, Ganoderma spp., Irpex lacteus, Dichomitus squalens, and Ischnoderma resinosum, in Basidiomycete class, were efficiently capable of decolorization of pulping
%U http://www.hindawi.com/journals/ijce/2010/162504/