%0 Journal Article
%T 5-Aza-C对人鼻咽癌细胞hTERT基因表达及端粒酶活性的影响
%A 张自雄
%A 戴梦源
%A 王燕
%A 肖伯奎
%A 陈始明
%A 陶泽璋
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.01.002
%X 摘要 目的 研究去甲基化药物5-氮杂胞嘧啶核苷（5-Azacytidine, 5-Aza-C）对鼻咽癌细胞亚端粒区D4Z4甲基化状态、hTERT基因表达及端粒酶活性的影响。方法 常规培养鼻咽癌CNE, CNE1，CNE2及5-8F细胞系。5-Aza-C处理鼻咽癌细胞后，甲基化测序聚合酶链反应（MSP）法检测亚端粒区D4Z4甲基化；RT-PCR检测hTERT mRNA水平；端粒重复序列扩增法（telomeric repeat amplification protocol,TRAP）法检测端粒酶活性。结果 5-Aza-C处理后，四种鼻咽癌细胞系的亚端粒区D4Z4序列的甲基化水平较处理前明显下降。2.5 μmol/L 5-Aza-C处理后，四种鼻咽癌细胞hTERT mRNA的表达明显下调，端粒酶活性显著抑制。 结论 在鼻咽癌的发生中，亚端粒区的DNA甲基化水平紊乱起了一定的作用，去甲基化药物5-Aza-C能下调hTERT表达，抑制端粒酶活性。探讨鼻咽癌细胞中hTERT表达与亚端粒区CpG岛甲基化状态之间的关系，可能为亚端粒区染色体结构异常在鼻咽癌发生中的作用提供深刻见解
%K 鼻咽癌
%K 5-Aza-C
%K 人端粒酶反转录酶
%K 端粒酶活性
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%U http://www.zlfzyj.com/CN/abstract/abstract8672.shtml