%0 Journal Article
%T RPL8基因修饰的树突状细胞对胶质瘤的免疫治疗作用
%A 何应中
%A 刘福慧
%A 谈艳芳
%A 黄俊琼
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.01.005
%X 摘要 目的 探讨RPL8基因修饰的树突状细胞（DC）对胶质瘤生长的抑制作用。方法 通过重组腺病毒将RPL8基因导入树突状细胞，荧光显微镜观察细胞内绿色荧光蛋白的表达，RT-PCR分析细胞内RPL8mRNA表达，RPL8基因修饰的DC与T细胞混合培养，MTT法检测活化T细胞对胶质瘤细胞的杀伤效应。DC注入荷瘤小鼠体内，观察肿瘤体积变化及小鼠生存时间。结果 重组病毒转染DC后，细胞内可见绿色荧光，RT-PCR分析细胞内有RPL8mRNA表达；Ad-RPL8组、Ad组及PBS组激活T细胞对GL261细胞的杀伤率分别为78%、49%和43%，Ad-RPL8组较Ad组及PBS组明显增高（P<0.05），RPL8基因修饰的DC注入小鼠后，与Ad组及PBS组比，小鼠生存期明显延长（P<0.05），肿瘤体积变小（P<0.05），肿瘤细胞坏死增多。结论 RPL8基因修饰的DC对胶质瘤具有生长抑制作用，为胶质瘤免疫治疗开辟新途径
%K 胶质瘤
%K 树突状细胞
%K RPL8
%K 免疫治疗
%K Anti-tumor Effect of Dentritic Cells Vaccine Sensitized by GnRH/M2 and mGM-CSF/βhCG Fusion Proteins on Prostatic Cancer in Mice
%K Effect of RNA Interference Decreasing MSI1 Expression on Chemosensitivity of Human Glioma Cells
%K Immune Killing Effect of Silencing TIPE2 Expression on T Lymphocytes Against Lung Adenocarcinoma Cells LA795
%K NSCLC的疫苗治疗研究进展
%K Effect of siRNA-mediated notch1 Gene Silencing on Apoptosis and Radiosensitivity of Glioma Cell Line U87-EGFRvⅢ
%K In vitro Investigation on Effect of Lentiviral Vector-mediated RNA Interference Inhibiting VASH1 Expression on Proliferation and Apoptosis of Human Glioma Cells U-87MG
%K Review on Radiation Dose Fractionation on High-grade Glioma Patients
%K LncRNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma
%K GM-CSF Enhances Effect of TMZ on High-grade Glioma Cells
%K Effects for Histone Deacetylase Inhibitors on Proliferation and MKK7 Expression in Glioma Cells
%K Comparison of rhG-CSF and rhGM-CSF Mobilization on Quantity and Function Change of Dendritic Cell-based Tumor Vaccine via Human Peripheral Blood Monocytes
%K Effect of HSP70 and iNOS in Process of Hyperthermia Reducing Glioma Invasion
%K Updates of Immunotherapy on Renal Cell Carcinoma
%K Research Status and Development of Adherens Junction Associated Protein-1 in Gliomas
%K Progress of Zoledronic Acid Combined with Targeted or Immunomodulatory Drugs in Antitumor Research
%U http://www.zlfzyj.com/CN/abstract/abstract8387.shtml