%0 Journal Article
%T 顺铂诱导人鼻咽癌CNE2细胞衰老及其相关基因表达谱的研究
%A 杨飞城
%A 蒋晓山
%A 阳晶
%A 黄岚珍
%A 黄希仕
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.01.002
%X 摘要 目的 探讨顺铂体外诱导人鼻咽癌CNE2细胞衰老的效应及其分子机制。方法 采用 MTT 比色法观察顺铂对CNE2细胞增殖的抑制作用，衰老相关-β-半乳糖苷酶（SA-β-gal）活性评判细胞衰老，流式细胞术分析细胞周期分布，实时定量PCR法检测78个人类细胞衰老相关基因的表达。结 果 顺铂对CNE2细胞的增殖具有明显抑制作用；1.0 mg/L顺铂处理后第6和第8天检测SA-β-gal染色阳性细胞数分别达到67.63%和89.22%，细胞阻滞于G2 期；TP53、TP63、p16Ink4a，p27Kip1、PTEN、RB1、Cdc25C、TBX3、Gadd45a、IGF1R、PIK3CA、BMI-1、B2M、MORC3、MYC和SPARC等16个衰老相关基因的mRNA水平显著升高（P＜0.05或0.01），Cyclin A2、Cyclin B1、Cyclin E1、CDK6、ATM、ETS2、MDM2、E2F1、ETS1、FN1、IGFBP3、RBL2、SERPINB2及SIRT1等14个基因表达水平下降（P＜0.05 或 0.01）。结论 顺铂能够在体外诱导CNE2细胞衰老，其机制涉及p16和p27介导的p53-pRB和PTEN衰老信号调控网络
%K 顺铂
%K 肿瘤细胞早衰
%K 鼻咽癌
%K 细胞周期阻滞
%K Clinical Analysis of Radiotherapy Combined with Cisplatin-based Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma
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%U http://www.zlfzyj.com/CN/abstract/abstract8384.shtml