%0 Journal Article
%T 热疗降低胶质瘤侵袭性及与HSP70和iNOS表达的关系
%A 孙月明
%A 张田
%A 彭海兵
%A 王银环
%A 秦丽娟
%A 贾永森
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.11.001
%X 摘要 目的 探讨热疗与热休克蛋白70（heat shock protein70, HSP70）及诱导型一氧化氮合酶（induced nitric oxide synthase, iNOS）表达的关系，阐明热疗降低胶质瘤侵袭性的作用及其可能机制。方法 热处理C6胶质瘤细胞后，酶联免疫吸附法和Western blot法动态检测胶质瘤细胞中HSP70抗体滴度和iNOS的表达水平；Griess法测定胶质瘤细胞中NO浓度；结晶紫染色法检测胶质瘤侵袭性的改变。结果 C6细胞经热疗后，HSP70抗体滴度增加，于30 min时最多（86.52 ng/L）。C6细胞内iNOS的表达水平也在热疗后明显增加，并于热疗60 min达高峰（IDV=228.31）后逐渐减少。热疗后胶质瘤细胞内NO浓度于热疗120 min时达高峰（OD=168.42），且NO浓度越高，胶质瘤侵袭性越低（n=8.21×106）。结论 胶质瘤细胞经热疗后HSP70表达水平增加，增加的HSP70可能通过某种机制提高了iNOS的表达水平，进而增加了胶质瘤细胞内的NO浓度而引起胶质瘤侵袭性的下降
%K An Update
%K Effects for Histone Deacetylase Inhibitors on Proliferation and MKK7 Expression in Glioma Cells
%K Research Status and Development of Adherens Junction Associated Protein-1 in Gliomas
%K Effects of Vascular Endothelial Cells on Invasion of Glioma Cells via Notch Signal Pathway
%K Retrospective Analysis of Treatment Situation in Gansu Province: 105 Cases of Glioma
%K MicroRNA-184 Expression in Glioma Tissues and Its Prognosis Value
%K Expression of Fatty Acid-binding Protein 5 and Cellular Retinoic Acid-binding Protein 2 in Gliomas
%U http://www.zlfzyj.com/CN/abstract/abstract8624.shtml