%0 Journal Article
%T miR-100对食管鳞癌细胞株Ec-109的抑制作用
%A 周韶梅
%A 曹敏军
%A 曾毅
%A 盛望
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.02.005
%X 摘要 目的 探讨miR-100对食管鳞癌细胞的调控作用。方法 构建携带GFP的miR-100表达质粒， 脂质体转染食管鳞癌细胞株Ec-109。分别利用流式细胞仪、细胞划痕和Transwell实验检测miR-100对细 胞周期、凋亡和迁移的调节作用。结果 在食管鳞癌细胞中高表达miR-100可诱导G1期阻滞，即停留于 G1期的细胞数量增加，进入S和G2/M期的数量减少；在无血清培养的条件下miR-100的高表达可促进细 胞凋亡同时抑制食管鳞癌细胞迁移。结论 miR-100在食管鳞癌细胞株Ec-109中高表达可诱导细胞周期 G1期阻滞、抑制细胞的迁移并促进其凋亡
%K miR-100
%K 食管鳞状细胞癌
%K 细胞周期
%K 细胞凋亡
%K 细胞迁移
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%U http://www.zlfzyj.com/CN/abstract/abstract8413.shtml