%0 Journal Article
%T 免疫组织化学与聚合酶链反应在结肠癌微卫星状态筛选中的比较
%A 万美珍
%A 刘永萍
%A 周林艳
%A 陈玉芳
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.12.013
%X 摘要 目的 比较免疫组织化学（IHC）与聚合酶链反应（PCR）检测结肠癌微卫星不稳定状态的一致性，评价IHC检测微卫星不稳定状态的可行性。方法 采用IHC和PCR两种方法分别检测80例结肠癌中微卫星不稳定状态。结果 （1）IHC法检测结果显示癌旁正常黏膜中错配修复蛋白（包括MLH1、PMS2、MSH2、MSH6）均阳性表达。四个蛋白表达缺失率分别为23.8%（19/80）、21.2%（17/80）、7.5%（6/80）、3.7%（3/80），其中有16例显示MLH1、PMS2表达同时缺失，3例显示MSH2和MSH6表达同时缺失。19例（23.8%）显示为高频微卫星不稳定，剩余病例显示微卫星稳定状态或低频微卫星不稳定；（2）PCR产物测序结果显示18例为高频微卫星不稳定，5例为低频微卫星不稳定，其余病例均为微卫星稳定状态；（3）两种方法具有高度一致性。结论 与PCR法相比，IHC具有操作简单、耗时短、费用低以及对实验仪器条件要求不高等优点，具有较高的临床应用价值，可以作为检测结肠癌微卫星不稳定状态的首选方法
%K 免疫组织化学
%K 聚合酶链反应
%K 结肠癌
%K 微卫星不稳定
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%U http://www.zlfzyj.com/CN/abstract/abstract8661.shtml