%0 Journal Article
%T 食管癌细胞线粒体中MYH9蛋白的分布及与SLP-2结合的研究
%A 何海蓉
%A 张旭
%A 张雪妍
%A 王跃锜
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.03.004
%X 摘要 目的 研究非肌性肌球蛋白重链9（myosin, heavy chain 9, non-muscle, MYH9）蛋白在食管鳞癌细胞线粒体内的分布，及MYH9蛋白与线粒体相关蛋白SLP-2的结合情况。方法 使用抗stomatinlikeprotein 2 （SLP-2）抗体，在食管鳞癌细胞系KYSE150细胞的裂解液里进行免疫共沉淀，共沉淀物使用MALDI-TOF-MS蛋白质质谱进行分析，以分析与线粒体相关蛋白SLP-2结合的分子。对分析结果再继续使用免疫共沉淀及GST pull down法进行验证。使用蔗糖密度梯度离心纯化分离细胞线粒体，利用Western blot明确MYH9在食管癌细胞线粒体内的分布。结果 在食管鳞癌细胞系KYSE150细胞的裂解液内，MYH9蛋白可与SLP-2蛋白共沉淀，并且MYH9蛋白也能与GST-SLP-2结合。MYH9蛋白既存在于KYSE150细胞分离纯化后的细胞质中，也存在于分离出的线粒体中。结论 MYH9在食管癌细胞内部分分布于线粒体中，并与线粒体相关蛋白SLP-2相互结合。MYH9可能影响食管癌细胞线粒体的功能
%K 非肌性肌球蛋白重链9
%K SLP-2
%K 线粒体
%K 食管鳞癌细胞
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%U http://www.zlfzyj.com/CN/abstract/abstract8437.shtml