%0 Journal Article
%T 人胃癌组织中CLIC4基因表达与微卫星不稳定性的关系
%A 王秋兰
%A 薛永杰
%A 韩涛
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.07.006
%X 摘要 目的 探讨微卫星DNA不稳定性在胃癌发生中的作用及其与CLIC4基因表达的关系。方法 采用PCR、非变性聚丙烯酰凝胶电泳及银染法检测40例胃癌组织及其相应的手术切缘正常组织中D1S234、D1S199、D1S507的微卫星不稳定性（MSI）；同时用免疫组织化学检测该40例及另外55 例胃癌组织中CLIC4的表达。结果 D1S234、D1S199和D1S507阳性率分别为25%（10/40）、27.5%（11/40）和5%（2/40）。各组织病理类型之间差异无统计学意义（P>0.05）。40例胃癌组织中，CLIC4在肿瘤实质阳性率为25%（10/40），肿瘤间质阳性率为67.5%（27/40），同时阳性率为15% （6/40），同时阴性率为12.5%（5/40）。Spearman等级相关分析显示D1S199与CLIC4肿瘤实质阳性率呈正相关（r=0.137, P=0.042），D1S507与CLIC4肿瘤间质阳性率呈负相关（r=-4.22, P=0.009）。95例胃癌组织中，CLIC4在肿瘤间质阳性率为72.6%（69/95），肿瘤实质阳性率为51.6%（49/95），同时阳性率为24.2%（23/95）。肿瘤间质阳性率明显高于肿瘤实质阳性率（χ2=8.945, P=0.003），肿瘤间质阳性病例主要为低分化及黏液腺癌，肿瘤实质阳性病例主要为高-中分化腺癌。随着肿瘤分化程度的降低，CLIC4间质阳性率增加。结论 D1S199与D1S507的MSI可能与胃癌的发生密切相关；CLIC4可能与胃癌的肿瘤间质成纤维化有关，促进了胃癌的演进
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%U http://www.zlfzyj.com/CN/abstract/abstract8791.shtml