%0 Journal Article
%T 慢病毒载体介导RNAi稳定抑制VASH1表达对人脑胶质瘤细胞增殖、凋亡影响的体外研究
%A 付锴
%A 刘细国
%A 江普查
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.03.005
%X 摘要 目的 探讨运用慢病毒载体介导的RNA干扰技术抑制血管生成抑制因子1（VASH1）的表达对人脑胶质瘤U-87MG细胞增殖和凋亡的影响。方法 应用pGCL-GFP质粒构建针对VASH1的慢病毒shRNA载体，转染入工具细胞293T，筛选出适合浓度的慢病毒转染人脑胶质瘤U-87MG细胞；通过RT-PCR和Western blot评价其对U-87MG细胞内VASH1表达的影响；用四甲基偶氮唑蓝（MTT）法观察U-87MG细胞增殖活性的改变；用流式细胞仪（FCM）检测U-87MG细胞凋亡的变化。结果 与转染阴性对照组和空白组相比，转染pGCL-GFP-VASH1慢病毒载体的U-87MG细胞VASH1的mRNA 蛋白表达水平明显降低（P<0.01）；细胞的增殖活性显著上调（P<0.01），细胞凋亡明显减少（P<0.01）。结论 VASH1 shRNA慢病毒载体可抑制VASH1在人脑胶质瘤U-87MG细胞中的表达，并增加肿瘤细胞的增殖活性，抑制细胞凋亡
%K VASH1
%K RNA干扰
%K 慢病毒载体
%K 胶质瘤
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%U http://www.zlfzyj.com/CN/abstract/abstract8710.shtml