%0 Journal Article
%T 乳腺癌外周血中分子检测标记的筛选
%A 叶云
%A 孙宇飞
%A 张倩
%A 钟英英
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.07.004
%X 摘要 目的 对乳腺癌患者和健康者血液样本的基因表达谱进行分析，从中发现检测乳腺癌的分子标记。 方法 以公共数据库GEO中表达谱数据GSE11545作为训练集，利用BRB-ArrayTools软件提取乳腺癌/正常血液样本的差异表达基因作为候选基因，选取两组间差异水平小于0.001的基因，通过复合变量预测、对角线线性判别分析、最邻近算法和支持向量机四种不同的方法对验证集GSE27562中的样本进行分类预测，留一法交叉验证计算错误分类率，ROC曲线评估预测结果。结果 训练集中乳腺癌与正常血液样本的显著差异基因为61个，从中筛选出39个基因作为分类器，四种不同的方法对验证集进行的分类预测准确率都基本达到甚至超过80%，ROC曲线下面积达到0.925，表明分类预测效果良好。结论 基因芯片分析可以筛选出外周血中乳腺癌的分子标记，有望为乳腺癌的早期临床检测提供一种新的方法
%K 乳腺癌
%K 外周血
%K 基因表达谱
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%U http://www.zlfzyj.com/CN/abstract/abstract8533.shtml