%0 Journal Article
%T ROR1对人肺癌A549细胞上皮-间质转化的影响
%A 于亮
%A 倪建林
%A 冯彦
%A 王作刚
%A 赵子梁
%A 邹俊卿
%A 黄洋
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1311
%X 摘要 目的 探讨受体酪氨酸激酶样孤儿受体-1（receptor tyrosine kinase-like orphan receptor，ROR1）诱导人肺癌A549细胞上皮-间质转化（epithelial-mesenchymal transition, EMT）的作用及可能机制。方法 构建ROR1慢病毒表达载体，感染A549细胞筛选稳定过表达ROR1的A549细胞，采用划痕实验、Transwell实验检测A549细胞的侵袭和迁移能力； real-time PCR、Western blot分别检测ROR1、EMT相关标志物的表达。结果 过表达ROR1能够促进A549细胞向间质样细胞表型转化，Western blot结果显示Vimentin、N-cadherin表达上调， E-cadherin表达下调；划痕实验、Transwell结果显示细胞侵袭迁移能力显著增强（P=0.0023）；Western blot结果显示过表达ROR1能够上调EMT转录因子Snail的表达，干扰Snail能够逆转ROR1诱导的EMT，即下调Vimentin、N-cadherin表达，上调E-cadherin表达；划痕实验、Transwell结果显示干扰Snail显著降低细胞侵袭迁移能力（P=0.013）；进一步研究发现ROR1通过激活AKT信号而促进Snail的表达。结论 ROR1能够促进人肺癌A549细胞EMT转化，其机制可能与ROR1调控AKT/Snail信号有关
%K A Meta-analysis
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%U http://www.zlfzyj.com/CN/abstract/abstract9188.shtml