%0 Journal Article
%T 阿霉素通过Stat3-cMyc途径诱导三阴性乳腺癌MDA-MB-468细胞耐药性
%A 史立宏
%A 孙秀宁
%A 崔镓钰
%A 张宝刚
%A 徐金媛
%A 成丛丛
%A 王丽华
%A 钟瑾怡
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0497
%X 摘要 目的 观察阿霉素(Adriamycin，ADM)对人三阴性乳腺癌MDA-MB-468细胞耐药性的诱导作用并探讨其机制。方法 培养人三阴性乳腺癌MDA-MB-468细胞，用不同浓度阿霉素处理细胞24 h后，MTT法检测细胞生长抑制率及细胞对阿霉素的敏感度。免疫荧光染色法检测耐药蛋白ABCG2的表达；免疫印迹法检测Stat3、p-Stat3、转录因子cMyc及耐药蛋白ABCG2的表达水平。结果 阿霉素持续刺激4周后，获得的MDA-MB-468/ADM细胞对阿霉素的敏感度明显降低，且耐药蛋白ABCG2的表达明显增加；MDA-MB-468/ADM细胞p-Stat3、cMyc及ABCG2的表达量显著增加；MDAMB-468/ADM细胞培养液中加入WP1066后Stat3磷酸化明显抑制，cMyc及ABCG2的表达水平下调，并且MDA-MB-468/ADM细胞对阿霉素的敏感度明显增强（P<0.05）。结论 阿霉素可以通过Stat3-cMyc途径诱导三阴性乳腺癌MDA-MB-468细胞产生耐药性
%K 三阴性乳腺癌
%K 阿霉素
%K 耐药性
%K Stat3
%K cMyc
%K ABCG2
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%U http://www.zlfzyj.com/CN/abstract/abstract9289.shtml