%0 Journal Article
%T TEC酪氨酸蛋白激酶在肝癌组织中高表达并参与肝癌耐药
%A 刘亚坤
%A 刘文
%A 方皓舒
%A 李菲菲
%A 汪思应
%A 钟明贵
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0107
%X 摘要 目的 研究TEC酪氨酸蛋白激酶在肝癌组织中的表达及其与肝癌耐药之间的关系，为肝癌临床治疗提供线索。方法 Real-time PCR、Western blot及免疫组织化学法检测癌旁、肝癌组织及肝癌细胞系中TEC mRNA、TEC蛋白表达水平；狭线杂交法检测肝癌组织中TEC mRNA表达水平；MTT检测细胞存活率。构建TEC真核表达载体，经脂质体介导法转染肝癌细胞；RNA干扰技术敲低肝癌细胞中TEC。免疫共沉淀技术检测TEC磷酸化活化水平。结果 18例肝癌组织中，TEC mRNA和蛋白水平显著高于癌旁组织（P<0.001）；48例肝癌组织中，TEC高表达组对化疗药物的耐受能力明显高于TEC低表达组；上调TEC表达水平后，HepG2细胞对化疗药物的耐受性增加；用化疗药物处理肝癌细胞株HepG2，其TEC表达水平增加，并伴随有ERK磷酸化水平升高。 结论 TEC在肝癌组织中的高表达促进了肝癌的耐药，且可能与ERK磷酸化水平升高有关
%K A Case Report
%K Knockdown of EN2 Induces Apoptosis and Enhances PTEN Protein Expression in Hepatocellular Carcinoma Cells
%K Comparison of Survival Rates Based on Recommended Treatment Algorithms of BCLC or HKLC Staging Systems for Hepatocellar Carcinoma Patients
%K Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
%K 3E10 Targeting CD24 Enhances Chemotherapy Sensitivity of Hepatocellular Carcinoma HuH-7 Cells
%K Comparison of Efficiency and Safety Between Radiofrequency Ablation and Stereotactic Body Radiotherapy on Primary Small Hepatocellular Carcinoma
%K Effect of MicroRNA-340 on Proliferation and Apoptosis of Hepatocellular Carcinoma Cell Lines
%K Effect of Cyclin G1 on Radiosensitivity of Hepatocellular Carcinoma HepG2 Cells and Its Mechanism
%K Apoptotic Effect of Quinalizarin on Human Hepatoma Huh7 Cells and Its Antitumor Mechanism
%U http://www.zlfzyj.com/CN/abstract/abstract9230.shtml