%0 Journal Article
%T 二甲双胍对人食管鳞癌侧群细胞的作用及机制
%A 王留兴
%A 邵凯迪
%A 陆士新
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.1200
%X 摘要 目的 探讨二甲双胍（metformin, Metf）对人食管鳞癌侧群（side population, SP）细胞的作用及机制。方法 通过流式细胞仪检测食管鳞癌细胞系SP比例及分选SP细胞，CCK-8法、平板克隆及成球实验检测Metf对SP细胞体外增殖的影响，Western blot法检测Metf对SP细胞相关基因蛋白表达的影响。结果 本实验中的9种食管鳞癌细胞系的SP比例在0.2%~2%左右。Metf能够降低KYSE 150 SP比例、明显抑制SP细胞的细胞增殖、克隆形成数和成球生长数，其抑制程度与Metf浓度及给药时间呈显著正相关性，差异有统计学意义（P<0.01）。此外，Metf能不同程度地降低SP细胞中相关“干性”基因SOX2与OCT4的表达。结论 Metf 能抑制食管癌细胞中SP细胞，可能为食管癌治疗与预防提供新的途径
%K 食管鳞癌
%K 二甲双胍
%K SP细胞
%K 干性
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%U http://www.zlfzyj.com/CN/abstract/abstract9311.shtml