%0 Journal Article
%T 小檗碱通过调控IGF-1R信号通路抑制食管癌细胞增殖和诱导细胞凋亡的实验
%A 朱小飞
%A 李赛赛
%A 豆巧华
%A 郭晓芳
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1500
%X 摘要 目的 检测小檗碱对食管癌细胞增殖、周期和凋亡的影响，并探讨其作用机制。 方法 MTT法检测细胞的增殖抑制作用；碘化丙啶（Propidium iodide, PI）染色法检测细胞周期进程；Annexin V-FITC和PI双染法结合流式细胞技术检测小檗碱的凋亡诱导作用；Western blot技术检测小檗碱对胰岛素样生长因子1受体（IGF-1R）的活化及下游主要信号分子AKT和p44/42MAPK（ERK）磷酸化水平的影响。结果 小檗碱在体外可浓度依赖性地抑制四种食管癌细胞系的增殖，且其IC50值与细胞表面IGF-1R的表达水平呈负相关。小檗碱可使细胞阻滞在G2/M期。细胞凋亡实验结果显示，小檗碱可浓度依赖性地诱导食管癌细胞发生凋亡，并且在相同浓度下KYSE450细胞（IGF-1R高表达）的凋亡率显著高于KYSE150细胞（IGF-1R低表达）。Western blot结果显示，小檗碱处理可显著抑制IGF-1R的磷酸化，并抑制AKT和ERK的活化，且抑制作用随着小檗碱浓度的增加而增强。结论 小檗碱可通过对IGF-1R及其介导的下游信号通路的调控来发挥抑制食管癌细胞增殖、阻断细胞周期进程以及诱导食管癌细胞凋亡的作用
%K 小檗碱
%K 食管癌细胞
%K 胰岛素样生长因子1受体
%K 细胞凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract9167.shtml