%0 Journal Article
%T 灵芝多糖对T24荷瘤裸鼠化疗效果及其免疫逃逸的影响
%A 傅德望
%A 刘奔
%A 盛玉文
%A 郭鹏荣
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.05.008
%X 摘要 目的 探讨灵芝多糖（ganoderma lucidum polysaccharide, GLP）对膀胱癌化疗效果的影响及 其机制。 方法 建立T24荷瘤裸鼠模型，分组给药后测定脾脏指数，利用流式细胞仪检测小鼠脾脏和 血液中相关淋巴细胞亚群所占比例，采用双抗体夹心（ELISA）法检测荷瘤小鼠血清IL-10、VEGF、 TGF-β、IL-2和TNF-α含量，应用反转录-聚合酶链反应（RT-PCR）和Western blot检测小鼠脾脏IL-2 和TNF-α的表达。 结果 在荷瘤裸鼠模型中，灵芝多糖（200 mg/kg）灌胃18 d后可显著增加脾脏指 数（P<0.001）；顺铂+灵芝多糖组中小鼠脾脏及血液中的淋巴细胞（CD4、CD8、NK及DC）都较 顺铂组提高，而髓源性抑制细胞(myeloid derived suppressor cell, MDSC）含量降低。通过ELISA检测 发现灵芝多糖能明显增加外周血液中相关免疫因子（IL-10、VEGF、TGF-β、IL-2和TNF-α）的含量 （P<0.05）。 RT-PCR及Western blot的结果也证实了在灵芝多糖可增强小鼠化疗后脾脏中IL-2和TNF-α 的表达。结论 灵芝多糖能通过提升脾指数，增加脾脏和外周血相关淋巴细胞及免疫因子的含量，并 通过增强血清中IL-2和TNF-α的表达来提高小鼠的免疫力，增强抗肿瘤作用，其机制可能与灵芝多糖 降低免疫逃逸功能相关
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%U http://www.zlfzyj.com/CN/abstract/abstract8488.shtml