%0 Journal Article
%T pcDNA3.1-CD74-ROS1重组质粒构建与表达对肺癌细胞增殖及迁移的影响
%A 吴亚州
%A 张蕾
%A 李佳
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1486
%X 摘要 目的 构建C-ros致癌基因1（ROS1）融合基因CD74-ROS1重组质粒，并转染A549细胞株，检测其对肺癌细胞迁移能力的影响。方法 通过PCR技术获得CD74和ROS1基因片段，利用融合PCR法构建CD74-ROS1融合基因，构建pcDNA3.1-CD74-ROS1重组质粒，通过脂质体转染技术转入A549细胞中，利用Real-time PCR和Western blot技术检测CD74-ROS1的表达。MTT法观察细胞增殖情况，结晶紫染色法观察细胞形态变化，划痕实验检测CD74-ROS1对肺癌细胞迁移能力的影响。结果 酶切及测序结果表明CD74-ROS1融合基因构建成功，阳性克隆的CD74-ROS1蛋白表达显著增加。CD74-ROS1过表达导致细胞增殖能力增强，细胞形态上发生上皮-间质样转变且细胞迁移能力增强。结论 pcDNA3.1-CD74-ROS1重组质粒构建成功，并在A549细胞中稳定表达，CD74-ROS1过表达能够促进肺癌细胞增殖、形态转变且增强其迁移能力
%K CD74-ROS1
%K 融合PCR
%K 非小细胞肺癌
%K 迁移
%K 增殖
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%K Migration and Invasion Abilities of Hepatoma Cells
%K Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells
%K Effects of miR-144 on Proliferation
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%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
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%K Proliferation and Apoptosis of Gastric Cancer Cells
%K Research Progress on Resistance Mechanisms and Overcoming Strategies to Thirdgeneration EGFR-TKIs in Advanced Non-small Cell Lung Cancer
%K Advances in Prognostic Value of Peripheral Blood Inflammatory Indexes in Non-small Cell Lung Cancer
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%K Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line
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%U http://www.zlfzyj.com/CN/abstract/abstract9133.shtml