%0 Journal Article
%T 结直肠癌中miR-31的表达及预测靶基因的生物信息学分析
%A 张丽静
%A 杜志坚
%A 王园园
%A 翟丛劼
%A 赵增仁
%A 韩晓东
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.10.012
%X 摘要 目的 检测microRNA-31（miR-31）在结直肠癌中的表达及功能，预测其靶基因并进行生物信息学分析，为研究其作用和调控机制奠定基础。方法 采用实时荧光定量反转录聚合酶链反应（qRT-PCR）检测8种结肠癌细胞株、40例结直肠癌（colorectal cancer, CRC）患者癌组织及匹配的正常黏膜组织及33例结直肠腺瘤组织中miR-31的表达情况，并分析癌组织中miR-31表达与患者临床特征的关系。MTS法观察转染miR-31模拟物（mimics）组、抑制剂（inhibitor）组和对照组（miRControl）及空白细胞组细胞生长的差异，Western blot检测空白细胞组、miR-31 mimics和inhibitor三组细胞PCNA蛋白的表达。TargetScan、DIANA-microT、miRanda等软件预测miR-31的靶基因，并进行KEGG功能和信号通路富集分析。结果 miR-31在8种结肠癌细胞株中高表达，同时CRC患者癌组织中的表达高于腺瘤及正常黏膜组织（P<0.05）。其中癌组织和匹配的正常黏膜相比，miR-31表达明显上调（P=0.035），但腺瘤和正常黏膜相比，差异无统计学意义（t=0.122, P=0.904）。miR-31的表达与临床病理特征间未见明显关系（P均＞0.05）。转染miR-31 mimics后，miR-31的表达明显上调且和miR-Control组及空白细胞组相比，miR-31 mimics组细胞生长加快；而转染miR-31 inhibitor组miR-31表达显著降低，细胞生长活力明显受抑制。同时转染miR-31 inhibitor组PCNA蛋白表达较miR-31 mimics组和空白细胞组显著降低。生物信息学分析miR-31靶基因功能集中于转录后及翻译水平的调节、细胞连接、迁移及细胞运动等生物学过程。信号通路主要富集于内吞作用、轴突导向、T细胞受体信号通路、Wnt及MAPK信号通路。结论 miR-31在结直肠癌中高表达，且miR-31可以促进细胞生长和增殖，其靶基因可能通过调节多种生物学过程发挥作用
%K 微小RNA31（miR-31）
%K 结直肠癌
%K 实时荧光定量反转录聚合酶链反应
%K 生物信息学
%K Expression and Correlation of Id-1
%K MMP-9 and LMVD in Colorectal Adenocarcinoma Tissues
%K Mortality Trend of Colorectal Cancer in Kunshan City
%K Jiangsu Province
%K 1981-2014
%K Recent Advances of Correlation of Mismatch Repair System hMutS
%K hMutL Variation and Colorectal Cancer
%K Bioinformatics Analysis of Genes Related to Multidrug Resistance in Ovarian Cancer
%K Research Progress of Association of Vitamin D3 and Colorectal Cancer
%K LncRNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma
%K Effect of Apolipoprotein E on Invasion and Migration of Colorectal Cancer Cells
%K Individualized Treatment for Advanced Colorectal Cancer Patients Based on Molecular Characteristics
%K Serum Peptidome Patterns of Colorectal Cancer Based on Magnetic Bead Separation and Mass-spectrometry Technique
%K Role of Cancer-associated Fibroblasts in Occurrence and Development of Colorectal Cancer
%K Role of Hepatocyte Growth Factor Receptor c-met Regulating Epithelial-mesenchymal Transition in Metastasis of Colorectal Cancer
%K Virulence Gene Mutation in Seven Kindreds of Lynch Syndrome in Yunnan Province
%K Meta-analysis of Relationship Between Blood 25-hydroxyvitamin D Level and Colorectal Cancer
%K Epidemiological Trends of Colorectal Cancer
%K Expression of KLF17 Protein in Human Colorectal Cancer Tissue and Its Correlation with Prognosis
%U http://www.zlfzyj.com/CN/abstract/abstract8611.shtml