%0 Journal Article
%T 外周血PTEN、RASSF1A甲基化及其mRNA表达与结节性甲状腺疾病的关系
%A 乌云
%A 庞春艳
%A 李永超
%A 王朝霞
%A 魏枫
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.07.007
%X 摘要 目的 探讨PTEN、RASSF1A基因启动子甲基化及其mRNA表达与结节性甲状腺疾病之间的关系。方法 选取46例结节性甲状腺肿、34例甲状腺腺瘤、24例甲状腺乳头状癌患者（甲癌）和30例健康体检者，利用甲基化特异性PCR检测外周血中PTEN、RASSF1A基因启动子区甲基化状态及利用反转录—多聚酶链反应法（RT-PCR）检测mRNA表达情况。结果 （1）腺瘤组和甲癌组中PTEN、RASSF1A甲基化率分别与对照组比较, 差异均有统计学意义（均P<0.05）。（2）腺瘤组和甲癌组中PTEN、RASSF1A基因mRNA表达阳性率分别与对照组比较, 差异均有统计学意义（均P<0.05）。（3）甲癌组中PTEN和RASSF1A基因甲基化率、mRNA表达与淋巴结转移有关（均P<0.05），与年龄、性别、结节大小无关。结论 甲状腺肿瘤外周血中存在抑癌基因PTEN、RASSF1A高甲基化改变进而导致其功能失活，从而在甲状腺肿瘤的发生发展过程中发挥重要作用
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%U http://www.zlfzyj.com/CN/abstract/abstract8792.shtml