%0 Journal Article
%T 莱菔硫烷抑制肺癌干细胞增殖的体外实验
%A 张弓
%A 李雪梅
%A 谢有科
%A 黄丁平
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.07.003
%X 摘要 目的 研究莱菔硫烷（sulforaphane, SFN）对肺癌干细胞增殖的影响及其机制。方法 采用MTT法分析SFN对H460细胞增殖的影响；应用流式细胞术（FACS）检测SFN对细胞凋亡和侧群细胞比例的影响；肿瘤球培养法分析SFN对肿瘤球生长的影响；使用shRNA慢病毒载体构建β-catenin低表达细胞株，并应用蛋白电泳法检测在β-catenin正常或低表达情况下莱菔硫烷对β-catenin、Oct4、Sox2、c-Myc、Nanog等基因表达的影响。结果 SFN有效抑制H460细胞增殖，IC50为11.2 μmol/L。SFN处理72 h后，细胞凋亡呈剂量依赖性增高。SFN有效抑制原代肿瘤球和2代肿瘤球的生长，在较低浓度（1.0 μmol/L）下即有明显的抑制作用。FACS检测提示侧群细胞比例随SFN浓度增高而减少。SFN浓度依赖性地抑制β-catenin、Oct4、Sox2、c-Myc和Nanog等蛋白表达。在低表达β-catenin情况下，Oct4、Sox2、c-Myc、Nanog等基因表达水平与SFN浓度相关。结论 SFN通过β-catenin和干性相关基因（Sox2、c-Myc、Nanog和Oct4）特异地抑制肺癌干细胞的增殖
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%U http://www.zlfzyj.com/CN/abstract/abstract8788.shtml