%0 Journal Article
%T miR-155/PTEN调控轴在肝癌中的表达及其机制
%A 徐钢
%A 闫茂生
%A 高轶
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.0931
%X 摘要 目的 检测microRNA-155（miR-155）在肝细胞癌中的表达并探讨其生物学作用及机制。方法 采用荧光定量PCR法检测miR-155在30对肝细胞癌和癌旁组织中的表达。在体外实验中，采用miR-155 mimic过表达miR-155，利用CCK-8和Transwell实验检测细胞增殖和迁移的变化。采用生物信息学和荧光素酶报告基因实验预测和验证miR-155的靶基因人第10号染色体缺失的磷酸酶及张力蛋白同源物基因（PTEN），并分析其生物学作用。应用cBioPortal数据库分析miR-155及PTEN对肝癌患者预后的影响。结果 miR-155在肝癌组织中较癌旁组织表达升高（P<0.0001），过表达miR-155能促进肝癌细胞的增殖和迁移。miR-155高表达患者的疾病无进展生存期（DFS）较低表达组显著降低（P=0.021）。miR-155直接负性调控PTEN的表达，并增强胞内磷脂酰肌醇激酶（PI3K）通路信号。PTEN表达缺失患者的疾病无进展生存期较PTEN表达正常组差（P=0.0133）。结论 miR-155/PTEN调控轴对肝癌的进展发挥促进作用
%K A Meta-analysis
%K Effect of Down-regulation of MLLT3/AF9 Expression on Proliferation and Invasion Abilities of Hepatoma SMMC-7721 Cells
%K Advances of CXCL12-CXCR4/CXCR7 Biological Axis in Hepatocellular Carcinoma
%K Effect of IL-4/IL-4R on Biological Behavior of Hepatocellular Carcinoma and Its Underlying Mechanism
%K Primary Hepatocellular Carcinoma with Isolated Sinonasal Metastasis: One Case Report and Literature Review
%K Expression of PTEN and EMMPRIN in Lung Adenocarcinoma Tissues and Their Correlation with EGFR Mutation
%K NDRG2 Inhibits Migration of Breast Cancer Cells Through Upregulating PTEN Expression
%K Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway
%K Effects of Silencing B7-H3 Gene on Invasion of Human Hepatocellular Carcinoma Cells
%U http://www.zlfzyj.com/CN/abstract/abstract9104.shtml