%0 Journal Article
%T 跨膜接头蛋白PAG1过表达对皮肤鳞癌细胞A431运动能力的影响
%A 张维娜
%A 李美佳
%A 毛凯平
%A 渠开攀
%A 王冰
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.09.002
%X 摘要 目的 研究跨膜接头蛋白PAG1过表达对人皮肤鳞状细胞癌A431运动能力的影响。方法 构建PAG1-EGFP融合蛋白的慢病毒表达载体，采用反转录病毒转染的方法建立PAG1过表达的A431细胞株，实验分为3组：亲本细胞组（未进行基因转染的A431细胞）、对照组（A431细胞转染仅含EGFP阴性对照病毒）、实验组（A431细胞转染PAG1-EGFP病毒）。流式细胞术检测细胞转染率，实时荧光定量PCR及Western blot检测转染后PAG1 mRNA及其蛋白表达，进一步验证细胞转染成功与否；利用划痕修复实验、Transwell迁移实验、侵袭实验三种不同的方法检测PAG1基因的过表达是否会对皮肤鳞癌细胞的生长、迁移和侵袭能力产生影响。 结果 实验组和对照组目的基因病毒的转染表达率分别为（94.97±0.15）%、（94.60±0.35）%；实验组细胞中PAG1基因mRNA的表达量约为亲本细胞组的1.6倍（P=0.000），转染后实验组中PAG1蛋白的相对表达水平明显增加（P=0.000），建立了稳定过表达PAG1的A431细胞系；PAG1过表达明显降低A431细胞愈合率（P=0.000）；实验组A431细胞迁移、侵袭能力明显降低（P=0.001, P=0.000）。结论 PAG1的过表达能抑制人皮肤鳞癌细胞运动能力，影响肿瘤的浸润及远处转移
%K 皮肤鳞癌
%K PAG1
%K 迁移
%K 侵袭
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%U http://www.zlfzyj.com/CN/abstract/abstract8825.shtml