%0 Journal Article
%T 过表达microRNA-7对人肺癌95D细胞凋亡的影响
%A 廖珍媛
%A 徐林
%A 李永菊
%A 李颖
%A 罗军敏
%A 陈超
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.02.002
%X 摘要 目的 探讨过表达microRNA-7对人肺癌95D细胞体内外凋亡的作用。方法 将miR-7真核表达载体（命名为p-miR-7）体外瞬时转染人肺癌95D细胞，利用TUNEL法观察95D细胞凋亡的变化；免疫荧光技术检测各组细胞磷酸化Caspase3和Caspase9蛋白的表达；建立人肺癌裸鼠模型，肿瘤局部注射p-miR-7后利用TUNEL法检测肿瘤局部细胞凋亡变化，同时，免疫组织化学法检测凋亡相关的磷酸化Caspase3和Caspase9蛋白的表达变化。结果 体外转染p-miR-7后可导致人肺癌细胞的凋亡（P<0.05）。同时，细胞中磷酸化Caspase3和Caspase9蛋白水平显著增加（P<0.05）；肿瘤局部注射p-miR-7后，肿瘤局部miR-7表达水平及细胞凋亡也明显增强（P<0.05），磷酸化Caspase3和Caspase9蛋白水平也显著增加。结论 过表达miR-7可以导致肿瘤细胞凋亡，这与凋亡相关蛋白Caspase3和Caspase9磷酸化水平增加有关
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%U http://www.zlfzyj.com/CN/abstract/abstract8410.shtml