%0 Journal Article
%T 基因芯片筛选多形性胶质母细胞瘤差异表达基因和通路
%A 彭翔
%A 曹成安
%A 王建祥
%A 石磊
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1403
%X 摘要 目的 利用基因芯片技术和生物信息学分析方法，筛选出多形性胶质母细胞瘤相关的核心基 因和信号通路，为寻找多形性胶质母细胞瘤早期诊断和靶向治疗潜在标志物提供依据。方法 从GEO数据库中获取多形性胶质母细胞瘤mRNA表达谱芯片原始数据，利用R软件分析得到明显差异表达基因（differentially expressed genes, DEGs），对DEGs进行功能注释（GO ontology）和KEGG信号通路（KEGG signaling pathway）富集，进一步构建蛋白质相互作用网络（protein-protein interaction network, PPI），筛选核心基因，最后利用TCGA肿瘤数据库进行验证。结果 通过Pearson聚类分析发现肿瘤和正常组织聚类区分明显，说明表达谱结果可靠；差异基因共2 142个，其中上调基因968个，下调基因1 174个；GO和KEGG富集结果显示，差异基因的功能主要涉及细胞周期、细胞分裂和增殖、突触传递等生物学功能和通路，通路网络分析表明MAPK信号通路起核心调控地位。通过构建PPI网络筛选出9个与GBM密切相关的核心基因，进一步利用TCGA肿瘤数据库验证，与芯片结果一致。结论 KEGG信号通路和核心基因可能揭示了多形性胶质母细胞瘤发生发展的分子机制，核心基因可能用作多形性胶质母细胞瘤的早期诊断的分子标志物和治疗靶点
%K 多形性胶质母细胞瘤
%K 生物信息学
%K 基因芯片
%K 差异表达基因
%K 诊断靶标
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%U http://www.zlfzyj.com/CN/abstract/abstract9185.shtml