%0 Journal Article
%T siRNA干扰KLF8表达对鼻咽癌细胞上皮间质转化的作用
%A 於席芳
%A 王钢胜
%A 胡文兵
%A 陈军
%A 陈曦
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.12.009
%X 摘要 目的 探讨下调KLF8的表达对鼻咽癌CNE1-LMP1细胞侵袭能力的影响及其机制。方法 通过脂质体转染法将KLF8 siRNA真核表达质粒稳定转染至鼻咽癌细胞株CNE1-LMP1。Western blot和RT-PCR法检测CNE1-LMP1细胞中KLF8、E-cadherin、N-cadherin蛋白和mRNA表达水平的变化。Transwell侵袭实验观察siRNA后对CNE1-LMP1细胞侵袭能力的影响。结果 KLF8 siRNA转染的CNE1-LMP1细胞株与其阴性对照NC-si组比较KLF8蛋白和mRNA表达水平均下调，证实稳转细胞株建立成功。KLF8 siRNA可上调CNE1-LMP1细胞中E-cadherin的表达，而下调N-cadherin的表达。Transwell侵袭实验显示siRNA干扰CNE1-LMP1细胞侵袭能力下降。结论 KLF8在鼻咽癌CNE1-LMP1细胞中高表达；通过siRNA下调KLF8的表达能干扰上皮间质转化（EMT）相关蛋白E-cadherin、N-cadherin的表达，阻断EMT过程，抑制CNE1-LMP1细胞侵袭转移
%K Kruppel样转录因子8
%K CNE1-LMP1细胞
%K RNA干扰
%K 上皮间质转化
%K 侵袭
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%U http://www.zlfzyj.com/CN/abstract/abstract8891.shtml