%0 Journal Article
%T rhG-CSF与rhGM-CSF动员人外周血单核细胞后DC疫苗数量及功能变化的比较
%A 丁蓉蓉
%A 张燕
%A 张闯
%A 江龙委
%A 艾月琴
%A 蔡凯
%A 贾绍昌
%A 高艳荣
%A 黄伟谦
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.11.008
%X 摘要 目的 探讨利用人外周血单核细胞制备树突状细胞（dendritic cell, DC）疫苗过程中，使用重组人粒细胞集落刺激因子（recombinant human granulocyte colony-stimulating factor，rhG-CSF）和重组人粒细胞巨噬细胞集落刺激因子（recombinant human granulocyte/macrophage colony-stimulating factor, rhGM-CSF）进行动员的临床应用可行性，并比较两者作为动员试剂的优劣。方法 对2011年8月至2013年12月中国人民解放军第81医院肿瘤生物治疗科进行DC疫苗治疗的恶性肿瘤患者134例进行随机分组，其中rhG-CSF动员者69例，rhGM-CSF动员者65例。rhG-CSF动员组在血细胞分离机采集前1天行rhG-CSF皮下注射3 μg/(kg·d)，rhGM-CSF动员组在血细胞分离机采集前1天行rhGMCSF皮下注射3 μg/(kg·d)。比较两组动员前后血常规变化的差异；分析两组采集终产品中单个核细胞（mononuclear cell, MC）数量及表型，检测体外培养所得DC的数量、表型及细胞因子的表达。结果 动员后rhGM-CSF动员组单核细胞增高值高于rhG-CSF动员组（P≤0.001），但中性粒细胞计数增高值低于rhG-CSF动员组（P≤0.001），其余血常规项目两者差异无统计学意义。rhGMCSF动员组采集终产品中单个核细胞数量高于rhG-CSF动员组（P=0.046），而CD14+单核细胞的比例两组差异无统计学意义。rhGM-CSF动员组最终诱导所得DC数量高于rhGCSF动员组（P=0.011），且DC细胞表面分子HLA-DR的表达高于rhG-CSF动员组（P=0.001），其余CD80、CD86，CD83、CD11C、CD54表达两组差异无统计学意义。两组DC细胞Th1型和Th2型细胞因子的表达差异都无统计学意义。结论 在用人外周血单核细胞制备DC疫苗的过程中，使用rhGM-CSF动员比rhG-CSF更有优势
%K 肿瘤
%K 树突状细胞疫苗
%K 重组人粒细胞集落刺激因子
%K 重组人粒细胞巨噬细胞集落刺激因子
%K 单核细胞
%K Perioperative Security Evaluation of Intra-operative Radiotherapy for Recurrent Bone and Soft Tissue Sarcomas Around Pelvis
%K Role of CD147 and Its Ligand CypA in Hepatoma Carcinoma Hepa1-6 Cells Escaping from Immune Surveillance of T cells
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%K Comparison of Application Value of Contrast Enhanced Ultrasound and Acoustic Radiation Force Impulse Imaging in Differential Diagnosis of Benign and Malignant Renal Tumors
%K Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism
%K Death of Malignant Tumors and Potential Years of Life Lost Among Residents in Shandong Province
%K 2010-2012
%K Cancer Incidence and Mortality in Cancer Registries in Hubei Province
%K 2012
%K Liver Cancer Incidence and Mortality Rates During Last 40 Years in Hebei Province
%K Molecular Classification of Common Solid Cancers
%U http://www.zlfzyj.com/CN/abstract/abstract8631.shtml